Determination of loganin in Pterocephalus hookeri by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for determination of loganin in Pterocephalus hookeri.</p><p><b>METHOD</b>The analysis was performed on a Welchrom C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water (1288) as the the mobile phase at a flow rate of 1 mL x min(-1) and the detection wavelength was 240 nm.</p><p><b>RESULT</b>The peak areas and injection amounts of loganin had a good linear relationship at the range of 0.06-1.2 microg. The average recovery was 97.0%, and the RSD was 1.4%.</p><p><b>CONCLUSION</b>The method is simple, accurate, and can be used for its quality control.</p>