Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase
Experimental & Molecular Medicine
;
: 187-192, 2000.
Artigo
em Inglês
| WPRIM
| ID: wpr-25125
ABSTRACT
Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species of HeLa cell mRNA was discovered by RT-PCR cloning. Southern blotting and PCR analysis of the HeLa cell genomic DNA showed that the 2.3 kb message was encoded by the lysosomal alpha-mannosidase gene. Sequence comparison of the cDNA with the corresponding genomic DNA indicated that the 2.3 kb message was generated by an unusual intra-exonic joining event.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Células HeLa
/
Éxons
/
Processamento Alternativo
/
DNA Complementar
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Lisossomos
/
Manosidases
Limite:
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2000
Tipo de documento:
Artigo
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