A preliminary study on the influence of human plasma exosomes-like vesicles on macrophage Wnt5A-Ca²+ pathway / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 404-407, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-251940
ABSTRACT
<p><b>OBJECTIVE</b>To study the influence of human plasma exosomes-like vesicles on the regulatory function of macrophages.</p><p><b>METHODS</b>The exosomes-like vesicles were purified from healthy donors plasma with a series of high-speed centrifugation and ultrafiltration. Macrophages were derived from cultured human blood monocytes. The molecular markers of macrophages were assayed by FACS. After cultured with exosomes-like vesicles, the changes of macrophages cytoplasma Ca(2+), and related genes and proteins were assayed by FACS, RT-PCR and Western Blot, respectively.</p><p><b>RESULTS</b>After cultured with exosomes-like vesicles, mean fluorescent intensity (MFI) of macrophages cytoplasma Ca(2+) was increased. The vesicles enhanced macrophages to express cytokines genes, the expression of IL-1β and TNF-α genes being increased by 0.85 and 1.69 times respectively at 2 h, and that of IL-6 gene 3.7 times compared with the control at 8 h. However, the vesicles inhibited the expression of macrophages IL-10 gene, had no influence on the Frizzled5 receptor expression and could induce CaMKII phosphorylation.</p><p><b>CONCLUSIONS</b>Exosomes-like vesicles can up-regulat macrophages expression of inflammatory cytokines genes, and increase the secretion of inflammatory cytokines by activating the Wnt5A-Ca(2+) signaling pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cálcio
/
Proteínas Proto-Oncogênicas
/
Sinalização do Cálcio
/
Proteínas Wnt
/
Exossomos
/
Proteína Wnt-5a
/
Ativação de Macrófagos
/
Macrófagos
/
Metabolismo
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2011
Tipo de documento:
Artigo
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