Cerebral pathological evaluation following neural stem cells intraventricular transplantation in neonatal rats with periventricular leukomalacia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 362-366, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-252078
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the brain pathological changes following exdogenous neural stem cells (NSCs) intraventricular transplantation in neonatal rats with periventricular leukomalacia (PVL), and to explore the feasibility of NSCs transplantation for the treatment of PVL in premature infants.</p><p><b>METHODS</b>NSCs were prepared from E14 embryonic rat brain. Two-day-old neonatal rats were randomly divided into six groups PVL, PVL+culture medium, PVL+NSCs, sham operation, sham operation+culture medium, and sham operation+NSCs (18-21 rats each group). Intraventricular transplantation of exdogenous NSCs was performed 72 hrs after PVL induction or sham operation. The cerebral pathological evaluation was undertaken by light microscopy 7, 14 and 21 days after transplantation.</p><p><b>RESULTS</b>The pathological changes in the cerebral white matter were gradually improved with the prolonged time after transplantation. After 21 days of transplantation, 50% of the cerebral white matter showed mild pathological changes and 50% of that showed severe pathological changes, with neuronal pathological scores of 1.28+/-0.86, in the untreated PVL group. In the PVL+NSCs group, 30% of normal white matter, 40% of mild and 30% of severe pathological changes in the white matter were observed, with neuronal pathological scores of 0.32+/-0.16, 21 days after transplantation. There were very significant differences in both of pathological changes in the cerebral white matter and neuronal pathological scores between the PVL and PVL+NSCs groups (x2=10.7, P<0.01; F=29.664, P<0.01).</p><p><b>CONCLUSIONS</b>Intraventricular transplantation of exdogenous NSCs can apparently improve cerebral white matter damage. It is suggested that intraventricular transplantation of NSCs is of a great potential feasibility for the treatment of PVL in premature infants.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Terapêutica
/
Leucomalácia Periventricular
/
Encéfalo
/
Distribuição Aleatória
/
Ratos Sprague-Dawley
/
Biologia Celular
/
Transplante de Células-Tronco
/
Animais Recém-Nascidos
/
Neurônios
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2008
Tipo de documento:
Artigo
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