Rapid determination of thiabendazole residue in barbary wolfberry fruit by ion exchange chromatography and fluorescence detection / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To developed a method for the determination of thiabendazole residues in barbary wolfberry fruit by ion exchange chromatography (IEC) with fluorescence detection.</p><p><b>METHOD</b>The samples were extracted with 0.1% H3PO4. Chromatographic analysis was performed on a Supelcosil LC-SCX (4.6 mm x 250 mm, 5 microm) column eluted with 0.1 mol x L(-1) KH2PO4 (pH 3.0)-acetonitrile (7030) and detection at lamdaex = 307 nm and lamdaem = 359 nm.</p><p><b>RESULT</b>Thiabendazole in measured samples was separated completely. The calibration curve was linear at the range of 0.0005-0.02 mg x L(-1) with good precision and accuracy.</p><p><b>CONCLUSION</b>The proposed method was satisfactorily applied to the analysis of thiabendazole residues in barbary wolfberry fruit.</p>