Expression of ASMase in alcoholic liver fibrosis in rats / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 920-923, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-252298
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of the lysosomal enzyme acid sphingomyelinase (ASMase) in alcohol-induced hepatic fibrosis using a rat model.</p><p><b>METHODS</b>The model of liver fibrosis was induced by administration of alcohol and high fat diet using 20 rats. Six rats given no alcohol and normal diet served as the control group. Real-time PCR, western blotting, and immunohistochemistry were used to evaluate fibrosis-related changes in the mRNA and protein expressions of ASMase.</p><p><b>RESULTS</b>The fibrotic liver tissues of the model rats showed significantly higher expression levels of ASMase than the non-fibrotic liver tissues of the control rats (P less than 0.05).</p><p><b>CONCLUSION</b>Expression of ASMase is increased in the fibrotic liver tissue of an alcohol-induced hepatic fibrosis rat model, suggesting that this lysosomal enzyme may contribute to development of this disease condition.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Esfingomielina Fosfodiesterase
/
Ratos Sprague-Dawley
/
Fígado
/
Cirrose Hepática Alcoólica
/
Cirrose Hepática Experimental
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2013
Tipo de documento:
Artigo
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