New designed HMBA agents as inducers of erythroleukemia cell differentiation / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 27-31, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-252440
ABSTRACT
<p><b>OBJECTIVE</b>Searching for more potent and less toxic HMBA-related agents.</p><p><b>METHODS</b>Human erythroleukemia cell K562, murine erythroleukemia cell (MEL) and its sub-line MEL DS19 were used as target cells to select a cell line which is the most sensitive to HMBA, then analyzed the activity of inducing differentiation of two new designed HMBA derivatives HMBPA [hexamethylenebi (3-pyridin) amide] and Co-HDTA (ethylenediaminetetra acetic acid cobalt) using cell biology, cytochemical and molecular biology techniques.</p><p><b>RESULTS</b>We found that the MEL DS19 cells were most sensitive to HMBA (benzidine positive, B+ approximately 76%). Co-HDTA can inhibit the growth of MEL DS19, but induces differentiation just in a small population (B+ 2% approximately 4.5%). Between 0.02 approximately 5 micromol/L, HMBPA induces 3% approximately 8% cells committed to differentiation with little inhibition of cell proliferation. 1 micromol/L HMBPA and 2 mmol/L HMBA together, can obviously increase the percentage of differentiated cell (B+ approximately 72%), inhibit DNA synthesis and accelerate beta-globin transcription.</p><p><b>CONCLUSION</b>The new HMBA derivatives may provide potential cancer differentiation inducers.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Piridinas
/
Globinas
/
RNA Mensageiro
/
Células Tumorais Cultivadas
/
Leucemia Eritroblástica Aguda
/
Diferenciação Celular
/
Divisão Celular
/
Química
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2002
Tipo de documento:
Artigo
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