Purification of arsenic-binding proteins in hamster plasma after oral administration of arsenite / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 32-37, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-252668
ABSTRACT
<p><b>OBJECTIVE</b>To purify the arsenic-binding proteins (As-BP) in hamster plasma after a single oral administration of arsenite (iAs(III)).</p><p><b>METHODS</b>Arsenite was given to hamsters in a single dose. Three types of HPLC columns, size exclusion, gel filtration and anion exchange columns, combined with an inductively coupled argon plasma mass spectrometer (ICP MS) were used to purify the As-BP in hamster plasma. SDS-PAGE was used to confirm the arsenic-binding proteins at each purification step.</p><p><b>RESULTS</b>The three-step purification process successfully separated As-BP from other proteins (ie, arsenic unbound proteins) in hamster plasma. The molecular mass of purified As-BP in plasma was approximately 40-50 kD on SDS-PAGE.</p><p><b>CONCLUSION</b>The three-step purification method is a simple and fast approach to purify the As-BP in plasma samples.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Arsênio
/
Sangue
/
Farmacocinética
/
Proteínas de Transporte
/
Administração Oral
/
Cromatografia Líquida de Alta Pressão
/
Arsenitos
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2013
Tipo de documento:
Artigo
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