The effect of TSPG in vivo on transplantation of neural stem cells in treatment of Parkinson's disease mouse / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To observe the effect for the model of PD and the transplantation of NSCs after injection of TSPG into mouse in advance.</p><p><b>METHODS</b>Firstly, we divided the mouse into 5 groups. For the group of 1-4, we established the model of PD with MPTP. For the group of 5, before the establishment of the model, we injected TSPG into mouse in advance for prevention. And then, we evaluated the effect by paralysis agitans score standard and praxiology marker. Secondly, we obtained the NSCs from the 7-12 week embryo cerebral cortex. Then we transplanted NSCs which pretreated by TSPG into the striatum of the 5 groups. After 60 days, we obtained the brain section, and detected the TH by ICC to analyse the differentiation status of NSCs.</p><p><b>RESULTS</b>The prevention of TSPG could decrease the neural cells damage by MPTP, and could protect the nervous system. After we transplanted NSCs into the striatum of Parkinson' s disease mouse, we found that for the group of 5, the paralysis agitans, auto-activity and memory function had the most distinct amelioration. And the number of dopaminergic neurons increased most transparently in brain section, and the neurons contact was the most enriched with the adjacent nervous cells.</p><p><b>CONCLUSION</b>TSPG can decrease the neural cells damage and can produce a marked effect in treatment of PD by transplanting NSCs invivo.</p>