The effects of protooncogene on oocyte maturation mediated by cytokines / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 74-79, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-252705
ABSTRACT
<p><b>AIM</b>The mechanisms of cytokines in regulating oocyte maturation is still little known. The present study attempt to investigate whether the protooncogene of c-erbB2, c-myb are involved in introducing of cytokines to regulate oocyte maturation.</p><p><b>METHODS</b>This research used mouse GV stage oocyte culture model in vitro and RT-PCR, Western blotting method to explore the effect of EGF, TNFalpha, ET-1 and NO on oocyte maturation; to analyze the c-erbB2 mRNA and c-myb mRNA expression and the phosphorylation of MAPK and cyclinB1 expression in oocytes affected by above cytokines.</p><p><b>RESULTS</b>EGF(10 microg/L) stimulated meiosis of oocytes significantly, the level of c-erbB2 mRNA, c-myb mRNA were increased, and promoted the phosphorylation of MAPK and cyclinB1 expression; TNFalpha (1 microg/L) and ET-1 ((10(-1) mol/L) had the results to EGF. Low dose of SNP (10(-5)mol/L) had no effect on oocyte maturation, but could significantly reverse the suppression of dbcAMP on oocyte maturation.</p><p><b>CONCLUSION</b>c-erbB2 and c-myb were involved in introducing of cytokines to regulate oocyte maturation, might be the middle link in connection of the cytokines with MAPK and MPF in regulation oocyte maturation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oócitos
/
Oogênese
/
Fisiologia
/
RNA Mensageiro
/
Células Cultivadas
/
Citocinas
/
Fator Promotor de Maturação
/
Fator de Necrose Tumoral alfa
/
Receptor ErbB-2
/
Proteínas Quinases Ativadas por Mitógeno
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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