Effects of cardioplegia with tetrodotoxin on intracellular sodium overload of ischemia/reperfusion cardiomyocytes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 23-26, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-252718
ABSTRACT
<p><b>AIM</b>To investigate the effects of polarizing cardioplegia solution with sodium channel inhibitor tetrodotoxin (TTX) on intracellular free Na+ concentration ([Na+]i) in isolated cardiomyocytes of rat.</p><p><b>METHODS</b>Ventricular myocytes with beating were isolated from adult rat hearts by enzymatic dissociation, randomly created in group base, group STH2 (contrast group of ischemia/reperfusion) and group TFX (treated group). Both Group STH2 and group TTX were arrested by St. Thomas No. 2 cardioplegia solution and TTX cardioplegia solution respectively, the arrest/re-beating cell model imitating MIRI being established, and imaged by laser scanning confocal microscopy (LSCM) for measuring [Na+]i of cardiomyocytes in different period. The morphology of cardiomyocytes was observed under the inverted microscope.</p><p><b>RESULTS</b>[Na+]i of cardiomyocytes in both group TTX and group STH2 after re-beating was higher than that in group base (P < 0.01), and [Na+]i in group TTX was lower than that in group STH2 (P < 0.01). During arrest, the elevation of [Na+]i in group TTX was lower than that in group STH2. During arrest, the elevation of [Na+]i in group TTX was lower than that in group STH2. Morphologically, after re-beating, the ratio of active cardiomyocytes with normal form in group TTX was higher than that in group STH2 (P < 0.01).</p><p><b>CONCLUSION</b>Contrast depolarized cardioplegia solution, TTX cardioplegia solution could alleviate ischemia reperfusion injury and intracellular Na+ overload of cardiomyocytes.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Sódio
/
Tetrodotoxina
/
Soluções Cardioplégicas
/
Traumatismo por Reperfusão Miocárdica
/
Hipóxia Celular
/
Células Cultivadas
/
Biologia Celular
/
Bloqueadores dos Canais de Sódio
/
Miócitos Cardíacos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2009
Tipo de documento:
Artigo
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