The assay of transcriptional activation effect of TR3 and deletion mutation in the yeast two hybrid system / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 504-507, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-252727
ABSTRACT
<p><b>AIM</b>To assay the transcriptional activation effect of TR3 and it's deletion mutation in yeast two hybrid system.</p><p><b>METHODS</b>The total length of TR3 and TR3/delta1-690 gene was amplified by PCR method and cloned into pGBKT7 vector. Bait vector of pGBKT7-TR3 and pGBKT7-TR3/delta1-690 was transformed into AH109 competence yeast. Then self activation of the recombination vector was tested by assay the activity of beta-galactosidae.</p><p><b>RESULTS</b>The pGBKT7-TR3 and pGBKT7-TR3/AM 690 vector was successfully constructed. The filter paper containing beta-galactosidae didn't changed to blue showed that the reporter gene wasn't activationed.</p><p><b>CONCLUSION</b>TR3 and TR3/delta1-690 hadn't the activity of transcriptional activation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Ativação Transcricional
/
Deleção de Sequência
/
Técnicas do Sistema de Duplo-Híbrido
/
Membro 1 do Grupo A da Subfamília 4 de Receptores Nucleares
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2008
Tipo de documento:
Artigo
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