Multi-component quantitative method of Chinese herbal additives based on fingerprint / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a full-quantified fingerprint method for analyzing Chinese herbal additives by HPLC.</p><p><b>METHOD</b>A HPLC in combination with PDA detector was applied with a phenomenex luna C18 (4.6 mm x 250 mm, 5 microm) column by gradient elution using acetonitrile-0.1% formic acid solution as the mobile phase. The flow rate was 1 mL x min(-1) and the detection wavelength was set at full spectrum scan.</p><p><b>RESULT</b>According to the selected chromatographic conditions, the full-quantified fingerprint of the Chinese herbal additive has good precision, reproducibility, stability and recovery.</p><p><b>CONCLUSION</b>The HPLC method developed for simultaneous determination of seven compounds is simple and valid. It can be used for quality evaluation and quality control of Chinese herbal additive and its processing products.</p>