Cloning and characterization of 3-hydroxy-3-methylglutary CoA reductase cDNA of Glycyrrhiza uralensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1275-1279, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-252927
ABSTRACT
<p><b>OBJECTIVE</b>To clone and analysis the sequence of 3-hydroxy-3-methylglutary CoA reductase (HMGR) cDNA from Glycyrrhiza uralensis.</p><p><b>METHOD</b>The primers were designed based on the conservative region of HMGR nucleic acids sequence from public database. The target gene was obtained from root of G. uralensis by use of homologous cDNA amplificati on and RACE technologies. The sequence alignment was performed using BLAST. The open reading frame was identified by use of the ORF Finder. The protein domains were defined by use of Prosite software. Clustal was used to conduct multiple amino acid sequence alignment and MEGA 5.0 was used to conduct the phylogenetic tree.</p><p><b>RESULT</b>The GuHMGR cDNA sequence was obtained contains 1 842 bp contains a 1 722 bp ORF, encoding 573 amino acids with 3-hydroxy-3-methylglutary CoA reductases family profile. Deduced amino acid sequence had 84% and 76% homology to the amino acid sequence of Pisum sativum, Medicago truncatula.</p><p><b>CONCLUSION</b>The cloning of 3-hydroxy-3-methylglutary CoA reductase (HMGR) cDNA will provide a foundation for exploring the function of HMGR in glycyrrhizin biosynthesis.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Proteínas de Plantas
/
Dados de Sequência Molecular
/
Química
/
Alinhamento de Sequência
/
Sequência de Aminoácidos
/
Classificação
/
Clonagem Molecular
/
DNA Complementar
/
Glycyrrhiza uralensis
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Artigo
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