Effects of LOX-1 on expression of adhesion molecules induced by ox-LDL in HUVECs / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 743-747, 2005.
Article
em Zh
| WPRIM
| ID: wpr-253073
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of oxidized low-density lipoprotein receptor 1 (LOX-1) on secretion of adhesive molecules mediated by ox-LDL in human umbilical endothelial cells (HUVECs).</p><p><b>METHODS</b>HUVECs with different concentration of ox-LDL (0, 10, 20, 50, 100 microg/ml) were incubated for 24 h, or HUVECs were pretreated with 250 microg/ml poly (I) or 250 microg/ml carrageenan for 2 h and then incubated with 50 microg/ml ox-LDL for another 24 h. Expression of LOX-1 was determined by realtime RT-PCR and Western blot. mRNA and protein of ICAM-1, VCAM-1 and E-selectin were examined by RT-PCR and Western blot respectively.</p><p><b>RESULTS</b>Incubation of HUVECs with ox-LDL (10-100 microg/ml) enhanced the expressions of LOX-1, ICAM-1 and E-selectin in a concentration-dependent manner (P < 0.01). On the contrary, ox-LDL did not affect the expression of VCAM-1 by HUVECs. The expression of LOX-1, ICAM-1 and E-selectin induced by ox-LDL were reduced in HUVECs pretreated with 250 microg/ml poly (I) or 250 microg/ml carrageenan for 2 h and then incubated with 50 microg/ml ox-LDL for 24 h. This showed that both poly (I) and carrageenan obviously decreased the expression of LOX-1, ICAM-1 and E-selectin induced by ox-LDL.</p><p><b>CONCLUSION</b>ox-LDL may upregulate the expression of LOX-1, ICAM-1 and E-selectin, and LOX-1 blocker may partly inhibit this upregulation. The results suggest that the expression of inflammatory molecules induced by ox-LDL in HUVECs is mediated by LOX-1.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Veias Umbilicais
/
RNA Mensageiro
/
Endotélio Vascular
/
Moléculas de Adesão Celular
/
Adesão Celular
/
Células Cultivadas
/
Molécula 1 de Adesão Intercelular
/
Molécula 1 de Adesão de Célula Vascular
/
Selectina E
/
Biologia Celular
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2005
Tipo de documento:
Article