New Animal Model of Renal Cell Carcinoma / 대한비뇨기과학회지

ABSTRACT

PURPOSE: This study was undertaker in order to develop and characterize a new murine renal cell carcinoma(RCC) model which may be closely analogous to the human RCC and which may serve as a basis for the evaluation of new therapeutic modalities. MATERIALS AND METHODS: The tumor being investigated, Rag cell line, arose spont- aneously as a renal adenocarcinoma in an inbred Balb/c mouse. Tumor cells were injected into subrenal capsule and injected intravenously and intraperitoneally to induce tumors. Among the orthotopically inoculated animals, some were performed a nephrectomy to mimic the situation mostly often encountered in the human. RESULTS: 1 Tumors were transferred to all animals where inoculated into subrenal capsule. 2. The growth rates of tumor-bearing mice were retarded compared to control mice. 3. Tumors grew exponentially when inoculated into subrenal capsule. The doubling time of tumor was approximately 13 days. 4. Spontaneous lung metastasis could be better induced in the subrenal capsule model(80%). Higher rate of lung metastasis was obtained by intravenous inoculation of tumor cells(85.7%). 5. After nephrectomy performed on 14 days following subrenal capsular Inoculation of tumor cells, lung metastasis developed spontaneously in 80% of cases. CONCLUSIONS: The behavior of Rag tumor following subrenal capsular inoculation mimics that of human RCC from the viewpoint of the formation of primary tumor mass on the kidney followed by the development of spontaneous metastases. This model can be used for the evaluation of the therapeutic erects both on the primary tumor as well as on the metastatic disease.