Establishment of a HEK293 cell line stably expressing human HCN2 gene / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 254-256, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-253172
ABSTRACT
<p><b>AIM</b>To create a model for studying ionic channels by means of the expressing human HCN2 and G418-resistant HEK293 cell lines established.</p><p><b>METHODS</b>pcDNA3-hHCN2 was transfected with Lipofectin2000 into HEK293 cell line. The transfected cells would be survived in the further culture medium containing G418 antibiotic as the hHCN2 gene could express a G418 resistant products. Whole-cell patch clamp investigated that hHCN2 gene was transfected into HEK293 cells.</p><p><b>RESULTS</b>The G418 resistant (600 ug/ml) HEK293 cell line was established successfully and whole-cell patch clamp recorded ionic currents of transfected hHCN2.</p><p><b>CONCLUSION</b>The G418 resistant HEK293 cell line was successfully established with transfection of plasmid pcDNA3-hHCN2 by Lipofectin, which might be useful for studying the relationship between the structure and function of cloned ionic channels.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Transfecção
/
Canais de Potássio
/
Expressão Gênica
/
Técnicas de Patch-Clamp
/
Células HEK293
/
Canais Disparados por Nucleotídeos Cíclicos Ativados por Hiperpolarização
/
Vetores Genéticos
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Genética
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Canais Iônicos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2006
Tipo de documento:
Artigo
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