Experimental study on human bone marrow mesenchymal stem cells transfected by SDF-1 cDNA / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 147-150, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-253363
ABSTRACT
This study was aimed to investigate the expression of SDF-1 mRNA in SDF-1 cDNA-modified human bone marrow mesenchymal stem cells (hBMSCs) before and after transfection. The hBMSCs were isolated, cultured and identified, the SDF-1-pIRES2-EGFP eukaryotic expressing vector was constructed, and then the hBMSCs were transfected with the vector encapsulated by lipofectamine 2000. The transfection efficiency was measured by observing the expression of green fluorescence protein and detecting the mRNA by RT-PCR. The results indicated that the expression of SDF-1 mRNA increased by about 20% after hBMSCs were transfected instantaneously by SDF-1-pIRES2-EGFP. It is concluded that SDF-1 cDNA eukaryotic expression vector can be instantly transfected into hBMSCs by lipofectamine 2000, but the efficiency was too low to obtain enough steady transferred hBMSCs. Other procedures should be trialed to improve the transfection efficiency.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Células da Medula Óssea
/
Transfecção
/
DNA Complementar
/
Biologia Celular
/
Quimiocina CXCL12
/
Células-Tronco Mesenquimais
/
Vetores Genéticos
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2008
Tipo de documento:
Artigo
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