Isolation and identification of hetisine-type alkaloids from Aconitum coreanum by high speed countercurrent chromatography / 药学学报

ABSTRACT

<p><b>AIM</b>To search for more bioactive compounds from the roots of Aconitum coreanum (Lèvl.) Rapaics.</p><p><b>METHODS</b>High speed countercurrent chromatography was successfully applied to the separation of alkaloids from Aconitum coreanum. The structures were elucidated by their physicochemical properties and spectroscopic analysis.</p><p><b>RESULTS</b>Two-phase solvent system composed of CHCl3-CH3OH-0.2 mol x L(-1) HCl (1033, volume ratio) was used in this experiment, eight alkaloids were obtained from the roots of Aconitum coreanum, which were identified as 2alpha-propionyl-11alpha,13beta-diacetyl-14-hydroxyhetisine (I), Guanfu base P (II), Guanfu base G (III), Guanfu base F (IV), Guanfu base Z (V), Guanfu base O (VI), Guanfu base A (VII), Guanfu base B (VIII).</p><p><b>CONCLUSION</b>Compound I is a new alkaloid, named Guanfu base R.</p>