Parallel catalytic hydrogen wave of acetylspiramycin caused by dissolved oxygen and its application / 药学学报

ABSTRACT

<p><b>AIM</b>To propose a novel polarographic method for the determination of acetylspiramycin (ASPM) is proposed.</p><p><b>METHODS</b>In 0.1 mol x L(-1) NH4Cl-NH3 x H2O (pH 8.9) buffer containing dissolved oxygen, ASPM yielded a sensitive parallel catalytic hydrogen wave with the peak potential of -1.63 V (vs SCE) by single sweep polarography.</p><p><b>RESULTS</b>The 2nd order derivative peak currents (i(p)") of the parallel catalytic hydrogen waves of ASPM showed a linear relationship with its concentrations in the range from 1.74 x 10(-3) microg x mL(-1) to 3.84 microg x mL(-1) (r = 0.9979, n=13). Its detection limit was 5.80 x l0(-4) microg x mL(-1) (3sigma) and RSD (n=13) was 1.24% at the concentration level of 0.871 microg x mL(-1).</p><p><b>CONCLUSION</b>The proposed method could be applied to the determination of ASPM in ASPM tablets.</p>