Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1099-1104, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-253523
ABSTRACT
<p><b>AIM</b>To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel.</p><p><b>METHODS</b>Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9984 genes and expressed sequence tags (ESTs).</p><p><b>RESULTS</b>Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol x L(-1) paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol x L(-1) (IC50) and 100 nmol x L(-1) paclitaxel treatment, respectively.</p><p><b>CONCLUSION</b>Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Neoplasias da Mama
/
Regulação Neoplásica da Expressão Gênica
/
Ciclo Celular
/
Paclitaxel
/
Apoptose
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Linhagem Celular Tumoral
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2005
Tipo de documento:
Artigo
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