Establishment of high-throughput drug screening model targeting retinoic acid receptor in cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 908-911, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-253543
ABSTRACT
<p><b>AIM</b>To screen new drug for the treatment of acute promyelocytic leukemia, psoriasis and acne, high-throughput drug screening cell models marked by green fluorescent protein (GFP) have been established.</p><p><b>METHODS</b>Eight repeats of retinoic acid response element (RARE) were synthesized and cloned into a GFP expression vector. This construct was stably transfected into cells in vitro. Stable and sensitive cell clones with high copy numbers of RARE were selected by retinoic acid (RA) using fluorescence-activated cell sorting (FACS).</p><p><b>RESULTS</b>A cell line has been chosen to be high-throughput drug screening cell model. This model was shown with low background, high sensitive and good reproducibility, and was convenient and inexpensive.</p><p><b>CONCLUSION</b>This drug screening cell model can be used for retinoic acid receptor target high-throughput drug screening.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plasmídeos
/
Tretinoína
/
Proteínas Recombinantes
/
Transfecção
/
Linhagem Celular
/
Receptores do Ácido Retinoico
/
Elementos de Resposta
/
Biologia Celular
/
Proteínas de Fluorescência Verde
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2005
Tipo de documento:
Artigo
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