Analysis of ephedrine and its metabolites in rat urine by HPLC-ESI-ITMSn / 药学学报
Acta Pharmaceutica Sinica
;
(12): 838-841, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-253557
ABSTRACT
<p><b>AIM</b>To estabilish a rapid and sensitive LC-ESI-ITMSn method for the identification of ephedrine and its main metabolites in rat urine.</p><p><b>METHODS</b>After optimizing the detection condition of LC-ESI-ITMSn chromatography and mass spectrometry by using a standard ephedrine, the ionization and cleavage rules of ephedrine in ESI-MS and ESI-MSn modes were summarized, and then serving as the basis for the metabolite analysis of ephedrine in rat urine. Rat urine samples of 0-48 h were collected after ig 10 mg x kg(-1) ephedrine, then the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC-ESI-ITMSn.</p><p><b>RESULTS</b>The structures of ephedrine metabolites were elucidated according to the changes of the molecular weights of the metabolites (deltaM) and their cleavage pattern in ESI-ITMSn. As a result, three phase I metabolites and the parent drug ephedrine were identified existing in rat urine, but no phase II metabolites were found.</p><p><b>CONCLUSION</b>The LC-ESI-ITMSn method is rapid and highly sensitive and sepecific, it is suitable for the identification of ephedrine and its metabolites in rat urine.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Urina
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Sensibilidade e Especificidade
/
Ratos Wistar
/
Espectrometria de Massas por Ionização por Electrospray
/
Efedrina
/
Metabolismo
/
Métodos
/
Peso Molecular
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS