The formation of transgenic mice with hemangiomas / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 355-357, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-253699
ABSTRACT
<p><b>OBJECTIVE</b>To set up the animal model of hemangioma by microinjecting the PyMT transgenic DNA.</p><p><b>METHODS</b>Constructing the transgenic PyMT gene, and microinjecting it into fertilized embryos which were transferred to pseudopregnant recipients then. Observing the phenotype of the newborn-mice, detecting the integration of transgenic DNA by PCR, and analyzing the histological morphon of the neoplasm of the mice.</p><p><b>RESULTS</b>The transgenic DNA was proved to be right and has been microinjected into 579 fertilized embryos, 62 mice were born. Within the 62 mice, one mouse was found being the phenotype of hemangioma. PyMT gene was expressed in the total DNA of the transgenic mouse by PCR.</p><p><b>CONCLUSION</b>It could be a good way to build animal model of hemangioma with transgenic PyMT DNA.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Camundongos Transgênicos
/
Antígenos Transformantes de Poliomavirus
/
Genética
/
Hemangioma
/
Microinjeções
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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