Relationships between periapical lesion and IL-1, TNF-alpha gene expression in rat / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 345-347, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-253702
ABSTRACT
<p><b>OBJECTIVE</b>To understand the effects of the IL-1, TNF-alpha cytokines on the pathogenesis of periapical lesions by investigating its gene expression in rat.</p><p><b>METHODS</b>The model was established by surgically exposing mandibular molar teeth and left open to permit infection from the oral environment. The SD rats were killed at 1, 2, 3, 4 weeks and mandibular molar teeth X-ray were taken. In situ hybridization was used to test IL-1alpha, IL-1beta, TNF-alpha mRNA expression in periapical area and the kinds of positive cells were identified. Using image analysis system analyzed gene expression semi-quantified.</p><p><b>RESULTS</b>IL-1alpha, IL-1beta and TNF-alpha mRNA were all expressed beginning at 1 week, peaked at 3 weeks, and declined somewhat at 4 weeks, but IL-1beta mRNA was expressed at much lower levels with the same kinetics (P<0.01). Most of the staining occurred in areas that had heavy inflammatory infiltrate, fibroblasts, or endothelial cells. There was a statistically significant correlations between the area of periapical lesion and the number of positively stained cells for IL-1alpha and for TNF-alpha (IL-1alpha r=0.875, P<0.001; TNF-alpha r=0.858, P<0.001), and between the number of positive cells for IL-1alpha and that of for TNF-alpha (r=0.969, P<0.001).</p><p><b>CONCLUSIONS</b>IL-1alpha and TNF-alpha genes are highly expressed in developing periapical lesions in rat, which supports the hypothesis that these two cytokines play a key role in pulpal and periapical pathogenesis, including the concomitant bone destruction.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Periodontite Periapical
/
Fisiologia
/
RNA Mensageiro
/
Interleucina-1
/
Fator de Necrose Tumoral alfa
/
Ratos Sprague-Dawley
/
Hibridização In Situ
/
Alergia e Imunologia
/
Genética
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2003
Tipo de documento:
Artigo
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