Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 281-287, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-253846
ABSTRACT
<p><b>AIM</b>To investigate wether the corresponding protein of mono-ADP-ribosyltransferase 3 (ART3) mRNA is expressed in human testes and, if so, whether the expression is cell type-specific.</p><p><b>METHODS</b>ART3 mRNA was determined in human testes and sperm by reverse transcription-polymerase chain reaction (RT-PCR). The glycosyl-phosphatidylinositol linkage of ART3 was shown by treating ART3-transfected HEK-293-T cells with phospholipase C. Fluorescent activated cell sorter (FACS)-analyses were used to detect ART3 on mature spermatozoa and immunohistological studies to detect the protein in testes.</p><p><b>RESULTS</b>ART3 protein was shown to be present in testes. It was found on spermatocytes only. It was absent from spermatogonia, spermatids and spermatozoa. The absence of ART3 from spermatozoa was confirmed by FACS-analysis. ART3 protein was detected neither within a seminoma nor on Leydig cells.</p><p><b>CONCLUSION</b>Here we show for the first time that ART3 protein is expressed in testes in particular on spermatocytes, indicating that ART3 exerts a specific function only required at a particular stage of spermatogenesis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Especificidade de Órgãos
/
Espermatócitos
/
Espermatozoides
/
Testículo
/
RNA Mensageiro
/
Transfecção
/
Linhagem Celular
/
ADP Ribose Transferases
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Proteínas Ligadas por GPI
Limite:
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Artigo
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