Study on relationship between hepatitis B virus DNA load and genotype with large envelope protein / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 348-350, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-254061
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relation between hepatitis B virus DNA load and genotype with the level of large envelope protein.</p><p><b>METHODS</b>Serum HBV DNA was quantitively detected by using real time polymerase chain reaction (RT-PCR). The LHBs were detected by using enzyme linked immuno sorbent assay (ELISA) and HBV markers were detected by time differentiate immunofluorescence assay in 140 serum samples collected from chronic hepatitis B patients.The genotypes of HBV were identified by DNA sequencing; and analyze their relationship.</p><p><b>RESULTS</b>There was no significant difference between positive rate of LHBs and that of HBV DNA in HBeAg negative and positive group (P > 0.05); The HBV LHBs absorbency was markedly correlated with the HBV DNA load ( R2 = 0.9267). The difference of HBV LHBs absorbency between HBV genotype B and C was not significant.</p><p><b>CONCLUSIONS</b>The close correlation between HBV LHBs absorbence and HBV DNA load illustrated that he level of serum LHBs can be used to estimate the state of HBV replication; and there is no relationship between HBV LHBs absorbency and genotypes. So HBV LHBs may be used as a new serological marker to detect HBV replication.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírion
/
Virologia
/
DNA Viral
/
Ensaio de Imunoadsorção Enzimática
/
Química
/
Proteínas do Envelope Viral
/
Vírus da Hepatite B
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Alergia e Imunologia
/
Genética
Limite:
Adolescente
/
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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