Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 287-289, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-254079
ABSTRACT
<p><b>OBJECTIVE</b>To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.</p><p><b>METHODS</b>TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.</p><p><b>RESULTS</b>TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.</p><p><b>CONCLUSION</b>HBX protein could be induced into mouse liver by TAT induced peptide.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Proteínas Recombinantes de Fusão
/
Expressão Gênica
/
Transativadores
/
Membrana Celular
/
Transporte Proteico
/
Proteínas de Fluorescência Verde
/
Escherichia coli
/
Produtos do Gene tat do Vírus da Imunodeficiência Humana
/
Proteínas Virais Reguladoras e Acessórias
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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