CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV-infected individuals / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the CD8+ cell noncytotoxic antiviral response (CNAR) to HIV in nosocomial HIV infected individuals, and reveal the relationship between the CNAR and CD4+ cell count.</p><p><b>METHOD</b>CD8+ cells from HIV-1 sero-positive individuals were separated by immunomagnetic beads and mixed with CD4+ cells at different CD8 CD4 cell input ratios (21, 11, 0.51 and 0.251). Reverse transcriptase (RT) activity of cocultured supernatant was tested and compared with negative control and the suppression rate of HIV-1 replication was measured.</p><p><b>RESULT</b>The average CD8CD4 cell input ratios to reach 80% suppression of HIV replication in the group with CD4 < 300/microl and CD4 > 300/microl were 2.41 and 1.31, respectively (P < 0.05).</p><p><b>CONCLUSION</b>CNAR activity in HIV infected individuals is associated with CD4+ cell count. The ability to suppress HIV replication in subjects with CD4 > 300 is stronger than those with CD4 < 300.</p>