The primary application of direct rapid immunohistochemical test to rabies diagnosis in China / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 168-170, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-254115
ABSTRACT
<p><b>OBJECTIVE</b>Evaluation of the direct rapid immumohistochemical test (DRIT) for laboratory surveillance of rabies.</p><p><b>METHODS</b>72 brain specimens of domestic dogs or patients collected from Guizhou, Guangxi, Hunan, Anhui, Jiangsu and Yunnan provinces were detected by conventional methods including Direct Fluorescent-antibody Assay (DFA) and Reverse Transcription Polymerase Chain Reaction (RT-PCR), and by DRIT which was newly developed in the Rabies Section of the Centers for Disease Control and Prevention in the United States. The sensitivity and specificity of DRIT were evaluated by compare of the three results. By analysis of the index including cost of experiment, technique requirement and so on, the advancement and applicability of DRIT were discussed.</p><p><b>RESULTS</b>Compared with DFA and RT-PCR, DRIT will be more applicable for laboratories with limited funds and weak techniques because of its lower cost needed and simpler techniques required while its sensitivity and specificity are equal to the other two methods.</p><p><b>CONCLUSION</b>DRIT is more valuable in rabies diagnosis and more applicable for extension and popularization in rabies laboratory surveillance in local CDC.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Raiva
/
Vírus da Raiva
/
Virologia
/
Encéfalo
/
Dados de Sequência Molecular
/
China
/
Epidemiologia
/
Prevalência
/
Técnica Direta de Fluorescência para Anticorpo
/
Diagnóstico
Tipo de estudo:
Estudo diagnóstico
/
Estudo de prevalência
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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