Genome analysis of a newly isolated enterovirus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 110-112, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-254128
ABSTRACT
<p><b>OBJECTIVE</b>To demonstrate molecular characterization of a newly isolated enterovirus.</p><p><b>METHODS</b>Virus were isolated from patient with unknown-causing disease and tested by reverse transcription-polymerase chain reaction (RT-PCR) and 5'3'RACE (rapid amplification of cDNA ends, RACE), in an attempt to obtain the sequence of this newly isolated enterovirus.</p><p><b>RESULTS</b>Sequence analysis showed that this newly isolated enterovirus shared 83%-94% nucleotide identity and 91%-100% amino acid identity with enterovirus 89. Phylogenetic analysis indicated that it was probably a new subtype of enterovirus 89.</p><p><b>CONCLUSION</b>This newly isolated enterovirus in the stool specimen from patient has the same serotype with enterovirus 89, but it was probably a new subtype of enterovirus 89.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
RNA Viral
/
Sequência de Bases
/
Sequência de Aminoácidos
/
Classificação
/
Genoma Viral
/
Clonagem Molecular
/
Análise de Sequência de DNA
/
Enterovirus
/
Fezes
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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