Epidemiological survey on the infection of hepatitis E virus among pigs in Henan province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 24-26, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-254152
ABSTRACT
<p><b>OBJECTIVE</b>To investigate hepatitis E virus (HEV) infection among pigs in Henan province.</p><p><b>METHODS</b>A total of 623 swine sera, collected from 5 districts, were divided into two groups, under 3-month of age and over 3-month of age. They were tested for HEV antigen and antibody by using ELISAs, respectively. The sera positive for HEV antigen were tested for HEV RNA with RT-PCR. The positive products of RT-PCR were cloned and sequenced.</p><p><b>RESULTS</b>The positive rates of anti-HEV antibody of the groups under 3-month and over 3-month of age were 90.27% and 92.55%, respectively, without statistical difference, while those of HEV antigen were 15.93% and 5.69%, respectively, with significant difference. The positive rates of anti-HEV antibody and HEV antigen were significantly different among different districts. HEV RNA was detectable in 5 of 47 HEV antigen positive samples. The sequence analysis showed that in 4 of 5 specimens the sequence belonged to genotype 4 while in the remaining one the sequence was genotype 1.</p><p><b>CONCLUSION</b>The prevalence rate of HEV infection in pigs was high in Henan province and the rate differed in different districts.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Suínos
/
Doenças dos Suínos
/
Virologia
/
RNA Viral
/
China
/
Epidemiologia
/
Vírus da Hepatite E
/
Hepatite E
/
Análise de Sequência de DNA
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2008
Tipo de documento:
Artigo
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