Effects of suplatast tosilate on airway inflammation and interleukin-5 in asthmatic rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 759-763, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-254206
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of suplatast tosilate (IPD) on the airway inflammation and expression of interleukin-5 in asthmatic rats.</p><p><b>METHODS</b>Fifty adult male Sprague-Dawley rats (4-week- old) were randomly assigned to five groups placebo control, untreated asthma, budesonide(BUD)-treated asthma , early or late IPD intervention group (n=10 rats each). Asthmatic mode was prepared by ovalbumin sensitizion and challenge. Inflammatory cells and the percentage of EOS were detected in bronchoalveolar lavage fluid (BALF). The lung tissues were removed to detect the lung histomorphology. Gene expression of IL-5 was measured by reverse transcription-polymerase chain reaction (RT-PCR). Levels of interleukin 5 (IL-5) in BALF were measured using ELISA.</p><p><b>RESULTS</b>The inflammatory cells and the percentage of EOS in BALF, IL-5 levels in BALF and IL-5 mRNA expression in the lung tissues were obviously higher in the untreated asthma group than the control group (P<0.05), while the parameters in the IPD or BUD-treated asthma groups were significantly lower than the untreated asthma group (P<0.05).</p><p><b>CONCLUSIONS</b>IPD treatment can alleviate airway inflammation in asthmatic rats, possibly through inhibiting IL-5 mRNA transcripts.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Asma
/
Compostos de Sulfônio
/
Interleucina-5
/
Ratos Sprague-Dawley
/
Sulfonatos de Arila
/
Usos Terapêuticos
/
Tratamento Farmacológico
/
Alergia e Imunologia
/
Eosinófilos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2014
Tipo de documento:
Artigo
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