Growth inhibitory effect of adriamycin conjugated to single-chain antibody on human lung adenocarcinoma in vitro / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 718-721, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-254262
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the growth inhibitory effect of adriamycin (ADM) conjugated to an anti-lung cancer single-chain antibody (ScFv) 2A7-1 on lung adenocarcinoma cell line A2 in vitro.</p><p><b>METHODS</b>2A7-1 cell culture medium was concentrated by ultra-filtration (with Amicon P10Z filter), and soluble ScFv was purified using RPAS purification kit. ADM was conjugated to 2A7-1 by glutaraldehyde. A(280) and A(490) of the conjugate 2A7-1-ADM were determined by spectrophotometry and the molar ratio of 2A7-1 to ADM was calculated. Immunoreactivity of the conjugate was detected by immunohistochemistry. Its growth inhibitory effect on lung adenocarcinoma cell line A2 was determined by colony formation assay in vitro.</p><p><b>RESULTS</b>The molar ratio of 2A7-1 to ADM was 13.2. The conjugate strongly reacted with A2 cell. Its growth inhibitory effect on A2 cells was 4 times as potent as ADM.</p><p><b>CONCLUSION</b>Adriamycin conjugated to anti-lung cancer single-chain antibody 2A7-1 has much higher cytotoxic activity than unconjugated adriamycin against human lung adenocarcinoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Região Variável de Imunoglobulina
/
Adenocarcinoma
/
Doxorrubicina
/
Química
/
Ensaio de Unidades Formadoras de Colônias
/
Imunoconjugados
/
Linhagem Celular Tumoral
/
Proliferação de Células
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2004
Tipo de documento:
Artigo
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