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EB virus encoded latent membrane protein 1 mediated expression of transcription factor Ets-1 in nasopharyngeal carcinoma cells / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 454-457, 2004.
Artigo em Chinês | WPRIM | ID: wpr-254296
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the expression of tanscription factor Ets-1 mediated by EB virus encoded latent membrane protein 1 (LMP1) in nasopharyngeal carcinoma (NPC) cells.</p><p><b>METHODS</b>LMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2) were used. Expression of LMP1 and Ets-1 was observed after induction with Doxycycline (Dox). Expression of Ets-1 mRNA and protein was detected by RT-PCR and Western blot, respectively. The phosphorylation level of Ets-1 protein was examined by co-immunoprecipitation. The DNA binding activity of Ets-1 was detected by electrophoretic-mobility shift assay (EMSA).</p><p><b>RESULTS</b>After induction with Dox in pTet-on-LMP1 HNE2 cells, to some extent, the expression of Ets-1 mRNA and protein, its phosphorylation level and DNA binding activity were increased with enhancement of LMP1 expression.</p><p><b>CONCLUSION</b>LMP1 induces transcriptional activation and expression of Ets-1 which may contribute to the development of NPC.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Fosforilação / Fisiologia / Fatores de Transcrição / Virologia / RNA Mensageiro / Carcinoma de Células Escamosas / Proteínas da Matriz Viral / Neoplasias Nasofaríngeas Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Fosforilação / Fisiologia / Fatores de Transcrição / Virologia / RNA Mensageiro / Carcinoma de Células Escamosas / Proteínas da Matriz Viral / Neoplasias Nasofaríngeas Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2004 Tipo de documento: Artigo