Structural analysis of 22 full-length hepatitis B virus genomes isolated from patients with hepatocellular carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 213-216, 2004.
Article
em Zh
| WPRIM
| ID: wpr-254339
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the characteristics of full-length Hepatitis B Virus (HBV) genomes isolated from patients with hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>The full-length HBV genomes from the serum of HBsAg positive HCC patients were amplified by PCR, and then sequenced and analyzed its structure.</p><p><b>RESULTS</b>Twenty-two full-length HBV DNAs were obtained from different patients of HCC. Phylogenetic analysis revealed that all HBV strains could be categorized into genotype B or C and serotype adr or adw2. Structural analysis showed that HBV obtained shared meaningful consensus mutations in B/T cell epitopes of surface and core antigens, transactivating domain of X protein and enhancer II/core promoter regions as compared to standard strains.</p><p><b>CONCLUSION</b>Genotype and gene mutation of HBV may be closely correlated with the carcinogenesis of HBV-related hepatocellular carcinoma.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Filogenia
/
Virologia
/
DNA Viral
/
Transativadores
/
Vírus da Hepatite B
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Regiões Promotoras Genéticas
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Genoma Viral
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Clonagem Molecular
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Carcinoma Hepatocelular
/
Alergia e Imunologia
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2004
Tipo de documento:
Article