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Effect of ERK1/2 on rat pulmonary artery smooth muscle cells Kv1.5 channel in the process of hypoxia / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 418-426, 2015.
Artigo em Chinês | WPRIM | ID: wpr-254999
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of ERK1/2 MAPK pathway on the expression of Kv1.5 channel, a voltage-gated potassium ion channel, in rat pulmonary artery smooth muscle cells (PASMCs) and its mechanisms during the process of hypoxia.</p><p><b>METHODS</b>The PASMCs derived from SD rats were cultivated primarily. The third to sixth generation of PASMCs were divided into 5 groups randomly (1) Normal group (N); (2) Hypoxic group (H); (3) Demethy sulfoxide(DMSO) group (HD); (4) U0126 group (HU) 10 micromol/L U0126; (5) Anisomycin group (HA) 10 micromol/L anisomycin. There were three dishes of cells in each group. The cells in normal group were cultured in normoxic incubator (5% CO2, 37 degrees C), the cells in other groups were added to 0.05% DMSO in the hypoxic incubator (5% CO2, 2% O2, 37 degrees C), all cells were cultured for 60 h. RT-PCR and Western blot were used to detected the espressions of Kv1.5 mRNA and protein in PASMCs.</p><p><b>RESULTS</b>Compared with N group, the expressions of Kv1.5 mRNA and protein in H, HD and HA groups were reduced significantly (P < 0.05); Compared with H group and HD groups, Kv1.5 mRNA and protein expressions in HU group were increased sharply (P < 0.05). Compared with the HU group, Kv1.5 mRNA and protein expressions in HA groups were significantly lower (P < 0.05).</p><p><b>CONCLUSION</b>Low oxygen reduced Kv1.5 mRNA and protein expressions, U0126 could resistant the Kv1.5 channel lower expression caused by hypoxia. Anisomycin had no significant effect on Kv1.5 channel expression under hypoxia, but the expression of Kv1.5 was still significantly lower than the normal oxygen group. These data suggest that hypoxia may cause hypoxic pulmonary hypertension by interfering ERK1/2 signaling pathway to inhibit Kv1.5</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxigênio / Artéria Pulmonar / RNA Mensageiro / Hipóxia Celular / Ratos Sprague-Dawley / Proteína Quinase 1 Ativada por Mitógeno / Sistema de Sinalização das MAP Quinases / Biologia Celular / Miócitos de Músculo Liso / Proteína Quinase 3 Ativada por Mitógeno Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxigênio / Artéria Pulmonar / RNA Mensageiro / Hipóxia Celular / Ratos Sprague-Dawley / Proteína Quinase 1 Ativada por Mitógeno / Sistema de Sinalização das MAP Quinases / Biologia Celular / Miócitos de Músculo Liso / Proteína Quinase 3 Ativada por Mitógeno Limite: Animais Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2015 Tipo de documento: Artigo