Effect of AAVC-I on the migration of human umbilical vein endothelial cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 407-410, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-255002
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of component I from agkistrodon acutus venomon (AAVC-I) the migration of human umbilical vein endothelial cells (HUVECs), and to elucidate the possible anti-angiogenic mechanism of AAVC-I.</p><p><b>METHODS</b>The effect of AAVC-I on the migration of HUVECs which was cultivated in vitro and treated with AAVC-1 at four concentrations 0, 20, 40, 80 microg/ml, was observed by methods of scratch wound-healing and Transwell assay. The expression level of mRNA and protein of P-selectin and intercellular cell adhension molecule-I (ICAM-1) were examined by RT-PCR and Western blot assay.</p><p><b>RESULTS</b>Compared with the blank group, the migration ability of HUVECs in each AAVE-I treated group was reduced in a dose-dependent manner, and the expression level of the mRNA and protein of P-selectin and ICAM-1 were decreased.</p><p><b>CONCLUSION</b>AAVC-I inhibits the migration of endothelial cell, which is acted by down-regulation of the expression content of mRNA and protein of P-selectin and ICAM-1.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
RNA Mensageiro
/
Regulação para Baixo
/
Movimento Celular
/
Células Cultivadas
/
Molécula 1 de Adesão Intercelular
/
Selectina-P
/
Venenos de Crotalídeos
/
Células Endoteliais da Veia Umbilical Humana
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2015
Tipo de documento:
Artigo
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