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Differentiation of transplanted mouse c-Kit+lin- bone marrow cells into hepatocytes in vitro / 南方医科大学学报
Journal of Southern Medical University ; (12): 567-569, 2006.
Artigo em Chinês | WPRIM | ID: wpr-255252
ABSTRACT
<p><b>OBJECTIVE</b>To ascertain whether mouse c-Kit(+)Lin- bone marrow cells have the potential of hepatic stem cells.</p><p><b>METHODS</b>c-Kit(+)lin- bone marrow cells were isolated and purified by magnetic-activated cell sorting (MACS) from BALB/C male donor mice, and immediately transplanted into age-matched BALB/C syngeneic female mice with 35-Gy total liver irradiation. The recipients were sacrificed 1 month after the transplantation for pathological observation of the liver morphology. The presence of Y-chromosome was examined in the liver cells of the recipient by in situ hybridization (ISH), and alpha-fetoprotein (AFP) and albumin in the cells were detected by immunohistochemistry.</p><p><b>RESULTS</b>The hepatocytes positive for Sry gene on Y-chromosome were identified 1 month after transplantation, and immunohistochemistry for AFP and albumin confirmed that the donor mice-derived cells were hepatocytes.</p><p><b>CONCLUSION</b>c-Kit(+)lin- bone marrow cells have the potential of hepatic stem cells, which can reside and differentiate into hepatocytes in the liver after transplantation. c-Kit(+)lin- bone marrow cells can be used as the source cells of cell transplantation for liver disease.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transplante / Imuno-Histoquímica / Alfa-Fetoproteínas / Distribuição Aleatória / Diferenciação Celular / Irradiação Corporal Total / Transplante de Medula Óssea / Proteínas Proto-Oncogênicas c-kit / Hepatócitos / Biologia Celular Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transplante / Imuno-Histoquímica / Alfa-Fetoproteínas / Distribuição Aleatória / Diferenciação Celular / Irradiação Corporal Total / Transplante de Medula Óssea / Proteínas Proto-Oncogênicas c-kit / Hepatócitos / Biologia Celular Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo