Differentiation of transplanted mouse c-Kit+lin- bone marrow cells into hepatocytes in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 567-569, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-255252
ABSTRACT
<p><b>OBJECTIVE</b>To ascertain whether mouse c-Kit(+)Lin- bone marrow cells have the potential of hepatic stem cells.</p><p><b>METHODS</b>c-Kit(+)lin- bone marrow cells were isolated and purified by magnetic-activated cell sorting (MACS) from BALB/C male donor mice, and immediately transplanted into age-matched BALB/C syngeneic female mice with 35-Gy total liver irradiation. The recipients were sacrificed 1 month after the transplantation for pathological observation of the liver morphology. The presence of Y-chromosome was examined in the liver cells of the recipient by in situ hybridization (ISH), and alpha-fetoprotein (AFP) and albumin in the cells were detected by immunohistochemistry.</p><p><b>RESULTS</b>The hepatocytes positive for Sry gene on Y-chromosome were identified 1 month after transplantation, and immunohistochemistry for AFP and albumin confirmed that the donor mice-derived cells were hepatocytes.</p><p><b>CONCLUSION</b>c-Kit(+)lin- bone marrow cells have the potential of hepatic stem cells, which can reside and differentiate into hepatocytes in the liver after transplantation. c-Kit(+)lin- bone marrow cells can be used as the source cells of cell transplantation for liver disease.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transplante
/
Imuno-Histoquímica
/
Alfa-Fetoproteínas
/
Distribuição Aleatória
/
Diferenciação Celular
/
Irradiação Corporal Total
/
Transplante de Medula Óssea
/
Proteínas Proto-Oncogênicas c-kit
/
Hepatócitos
/
Biologia Celular
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo
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