Detection of mdr1 gene by real-time fluorescence quantitative polymerase chain reaction using Taq Man-MGB probe / 南方医科大学学报
Journal of Southern Medical University
;
(12): 466-468, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-255283
ABSTRACT
Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061 x 10(3) to 3.061 x 10(9) cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Membro 1 da Subfamília B de Cassetes de Ligação de ATP
/
Genes MDR
/
Taq Polimerase
/
Corantes Fluorescentes
/
Fluorometria
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo
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