In vitro killing effect of adenovirus-mediated fusion gene system driven by KDR promoter on gastric cancer cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 402-405, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-255300
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the killing effect of adenovirus(Ad)-mediated double suicide gene driven by kinase domain-containing receptor (KDR) promoter on gastric cancer MGC-803 cells.</p><p><b>METHODS</b>The 293 packaging cells were transfected by the plasmids pAdEasy-KDR-CDglyTK to generate infectious viruses. The gastric cancer MGC-803 cells were infected by the Ad followed by treatment with 5-FC and/or ganciclovir at different concentrations. The cell-killing effects were evaluated and the bystander effects analyzed after coculture of the cells without AdKDR-CDglyTK infection with the infected cells at different ratios. The cell cycle distribution was detected by flow cytometry and the pathological changes of the cells were observed by electron microscopy.</p><p><b>RESULTS</b>The infection rate of the resultant recombinant Ad in the cells increased gradually with increment of the multiplicity of infection (MOI) of the Ads. The killing effect of CD/TK fusion gene on the MGC-803 cells was much stronger than that of either of the single suicide gene (P<0.001), and considerable bystander effect was observed. The Ad infection caused MGC-803 cell growth arrest at G(1) phase with onset of apoptotic and necrotic morphologies of the cells as seen under electron microscope.</p><p><b>CONCLUSION</b>The CD/TK fusion gene system driven by the KDR promoter possesses effective killing effect on the KDR-expressing gastric cancer MGC-803 cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Neoplasias Gástricas
/
Proteínas Recombinantes de Fusão
/
Terapia Genética
/
Adenoviridae
/
Regiões Promotoras Genéticas
/
Receptores Proteína Tirosina Quinases
/
Linhagem Celular Tumoral
/
Citosina Desaminase
/
Genes Transgênicos Suicidas
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS