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Human cytomegalovirus induces apoptosis of ECV304 endothelial-like cells / 南方医科大学学报
Journal of Southern Medical University ; (12): 316-320, 2006.
Artigo em Chinês | WPRIM | ID: wpr-255323
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanisms for the cytopathic effect (CPE) of human cytomegalovirus (HCMV) in ECV304 endothelial-like cells.</p><p><b>METHODS</b>PCR and indirect immunofluorescence were used to detect HCMV infection by examining immediate-early (IE) gene and protein expression of the virus in ECV304 cells. Phase-contrast and electron microscopies were performed to observe the morphological changes of the infected and uninfected cells, and DNA ladder analysis and flow cytometry were carried out to study HCMV-induced cell apoptosis.</p><p><b>RESULTS</b>In HCMV-infected ECV304 cells, cytopathic effects were first observed at approximately 72 h post-infection. The cells with CPE changes exhibited detachment from the monolayer, cell rounding and shrinkage. The expression of the IE gene was detected. Chromatin condensation and nuclear fragmentation along with dramatic changes of the mitochondria were observed by electron microscopy at 96 h post-infection. Cellular DNA fragmentation was observed in the infected cells, which had cells apoptotic rates of 4.1% and 45.7% at 96 h and 144 h post-infection, respectively.</p><p><b>CONCLUSION</b>HCMV can induce apoptosis of ECV304 endothelial-like cells.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Veias Umbilicais / Virologia / DNA Viral / Microscopia Eletrônica / Linhagem Celular / Reação em Cadeia da Polimerase / Microscopia de Contraste de Fase / Apoptose / Proteínas Imediatamente Precoces Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Veias Umbilicais / Virologia / DNA Viral / Microscopia Eletrônica / Linhagem Celular / Reação em Cadeia da Polimerase / Microscopia de Contraste de Fase / Apoptose / Proteínas Imediatamente Precoces Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2006 Tipo de documento: Artigo