Sensitization of human glioma SWO cell line to tumor necrosis factor-induced apoptosis by blocking phospholipase C-gamma1 signaling pathway / 南方医科大学学报
Journal of Southern Medical University
; (12): 266-269, 2006.
Article
em Zh
| WPRIM
| ID: wpr-255336
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of phospholipase C-gamma1 (PLC-gamma1) in tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis of human glioma SWO cells.</p><p><b>METHODS</b>The PLC-gamma1 pathway was blocked by U73122 in SWO cells, and the inhibitory effect of TNF-alpha on SWO glioma cell proliferation with or without U73122 treatment was investigated by MTT assay. The cell apoptosis induced by TNF-alpha along or in combination with U73122 was detected by flow cytometry with PI staining. The expression of caspase-3 and Bcl-2 was detected by Western blotting.</p><p><b>RESULTS AND CONCLUSION</b>U73122 can sensitize SWO glioma cells to TNF-alpha-induced apoptosis. Blocking the PLC-gamma1 pathway may not induce apoptosis of SWO glioma cells, but can sensitize SWO glioma cells to small-dose TNF-alpha-induced apoptosis, the mechanism of which may involve down-regulation of bcl-2.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Inibidores de Fosfodiesterase
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Pirrolidinonas
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Transdução de Sinais
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Regulação para Baixo
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Sobrevivência Celular
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Western Blotting
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Fator de Necrose Tumoral alfa
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Apoptose
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Article