Effects of two variants of ING1 expression on tumor cell growth regulation / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 48-51, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-255357
ABSTRACT
<p><b>OBJECTIVE</b>To study effects of alternative transcripts of ING1 transfection on human cancer cell lines.</p><p><b>METHODS</b>p47/ING1A and p33/ING1B expression vehicles were constructed and introduced into a human breast cancer cell line MCF-7 and a human lung cancer cell line PAa, both expressing wild-type p53 protein. Growth characteristics of the transfectants and potentially related genes were analyzed.</p><p><b>RESULTS</b>The levels of p47/ING1A and p33/ING1B protein elevated respectively in tumor cells of MCF-7 and PAa after transfected with p47/ING1A and p33/ING1B, and the latter was much higher than that of the former. Ectopic overexpression of p33/ING1B effectively blocked tumor cell growth and arrested cells in the G(0) approximately G(1) phase of the cell cycle (P < 0.01), while p47/ING1A gave no effect on cell growth or cell cycle. Tumor cells overexpressing p33/ING1B contained more p21(WAF1) protein than that of the control cells, with undisturbed p53 protein level.</p><p><b>CONCLUSIONS</b>Expression of two different transcripts of ING1 may have different effects on tumor cell growth. p33/ING1B may cooperate with p53 in stimulating expression of p21(WAF1) gene, thus to arrest cell cycle and to inhibit tumor cell growth. p33/ING1B may be considered to be a candidate as a partner of p53 in gene therapy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Biossíntese de Proteínas
/
Neoplasias da Mama
/
Proteínas Nucleares
/
Transfecção
/
Adenocarcinoma
/
Proteínas
/
Ciclo Celular
/
Divisão Celular
/
Genes Supressores de Tumor
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2003
Tipo de documento:
Artigo
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