Application of serum protein markers to distinguish familial adenomatous polyposis (FAP) and sporadic colorectal adenomas / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 192-195, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-255532
ABSTRACT
<p><b>OBJECTIVE</b>To screen out specifically-expressed serum protein markers in familial adenomatous polyposis (FAP) and to establish a serum protein fingerprint diagnostic model for distinguishing FAP from sporadic colorectal adenomas.</p><p><b>METHODS</b>Serum samples were collected from 19 FAP cases and 16 sporadic colorectal adenomas with informed consent. Serum protein fingerprint profiles were detected by SELDI-TOF-MS with CM 10 protein chip to screen out FAP adenoma-related serum protein markers, and support vector machine (SVG) technique was used to establish the diagnostic model to distinguish FAP from sporadic colorectal adenomas.</p><p><b>RESULTS</b>Six differently-expressed protein peaks (P < 0.01) were detected. Among them proteins of 5640, 3160, 4180 and 4290 m/z were highly expressed in FAP adenomas, and proteins of 3940 and 3400 m/z were highly expressed in sporadic colorectal adenomas. The accuracy of diagnostic model established with SVG to distinguish FAP adenomas and sporadic colorectal adenomas was 94.7% and 93.7%, respectively.</p><p><b>CONCLUSION</b>SELDI-TOF-MS can be effectively used to screen out the differentially expressed serum protein markers in FAP adenomas and sporadic colorectal adenomas, and a diagnostic model build by SVG to distinguish them has been successfully established. Therefore, a useful breakthrough point for research on molecular mechanisms of FAP pathogenesis is provided.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Colorretais
/
Biomarcadores Tumorais
/
Adenoma
/
Polipose Adenomatosa do Colo
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Perfilação da Expressão Gênica
/
Análise Serial de Proteínas
/
Proteômica
/
Diagnóstico Diferencial
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2009
Tipo de documento:
Artigo
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