Expression of transcription factor T-bet and GATA-3 in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 189-191, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-255533
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of transcription factors (TF) T-bet and GATA-3 mRNA in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients.</p><p><b>METHODS</b>Sixty patients were divided into two groups according to the clinical data group A consisting of stage I and II, group B including stage III and IV. The gene expression of T-bet and GATA-3 in 60 esophageal cancer patients and 30 healthy controls was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of IFN-gamma and IL-4 was measured by enzyme linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>Expression of T-bet mRNA in esophageal cancer patients (stage I and II 0.27 +/- 0.05 ng/L, stage III and IV 0.12 +/- 0.02 ng/L) was significantly lower than that in the healthy controls (1.35 +/- 0.14 ng/L), but the expression of GATA-3 mRNA in esophageal cancer patients (stage I and II 0.45 +/- 0.06, stage III and IV 0.55 +/- 0.03) was significantly higher than that in the healthy controls (0.09 +/- 0.10). The plasma level of Th1 cytokine IFN-gamma in the patients [stage I and II (12.12 +/- 1.48) ng/L, stage III and IV (8.44 +/- 0.90) ng/L] was significantly lower than that in the healthy controls, while the level of Th2 cytokine IL-4 in the patients [stage I and II (18.64 +/- 0.77) ng/L, stage III and IV (25.28 +/- 2.02) ng/L] was significantly higher than that in the healthy controls. However, neither in the expression of T-bet and GATA-3, nor in the plasma level of IFN-gamma and IL-4, showed a significant difference between group A and B.</p><p><b>CONCLUSION</b>In the peripheral blood of esophageal cancer patients, the expression of T-bet decreased, while GATA-3 increased, Th1/Th2 balance is broken, and the Th2 is dominant. T-bet and GATA-3 play a part role in the regulation of Th1/Th2 balance.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Sangue
/
RNA Mensageiro
/
Ensaio de Imunoadsorção Enzimática
/
Neoplasias Esofágicas
/
Leucócitos Mononucleares
/
Interleucina-4
/
Interferon gama
/
Células Th2
/
Células Th1
Limite:
Adulto
/
Idoso
/
Aged80
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2009
Tipo de documento:
Artigo
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