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Effects of inhibiting SDF-1 expression by RNA interference on adhesion and drug sensitivity of Jurkat cells co-cultured with bone marrow stromal cells / 中华血液学杂志
Chinese Journal of Hematology ; (12): 458-460, 2005.
Artigo em Chinês | WPRIM | ID: wpr-255861
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of inhibiting stromal cell derived factor-1 (SDF-1) expression by RNA interference (RNAi) on adhesion and drug sensitivity of Jurkat cells co-cultured with bone marrow stromal cells.</p><p><b>METHODS</b>SDF-1 specific short hairpin RNA (shRNA) expressing plasmid was transferred into cultured human acute leukemic bone marrow stromal cells, positive clones were isolated by screening G418 resistance (Group A) , SDF-1 protein level in culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA). The adhesion rates to bone marrow stromal cells layer and the drug sensitivity to doxorubicin of co-cultured Jurkat cells were detected by cell counting and MTT assay, respectively. The un-transfected bone marrow stromal cells of acute leukemia patient (Group B) or normal subject (Group C) were taken as control.</p><p><b>RESULTS</b>The level of secreted SDF-1 protein (pg/10(5) cells/week) in the supernatants of Group A, B and C were 1920 +/- 205, 12,370 +/- 1355 and 6620 +/- 770, respectively. Of co-cultured Jurkat cells in Group A, B and C, the adhesion rates after 24 h co-culturing were (28.8 +/- 2.6)%, (57.4 +/- 3.8)% and (45.2 +/- 4.0)%, respectively, and the IC50 values of doxorubicin were 585, 6162 and 1758 nmol/L, respectively.</p><p><b>CONCLUSION</b>Down-regulating SDF-1 expression of bone marrow stromal cells by RNAi reduces adhesion rates and enhances drug sensitivity to doxorubicin of their co-cultured Jurkat cells.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células da Medula Óssea / Expressão Gênica / Adesão Celular / Células Cultivadas / Células Estromais / Resistencia a Medicamentos Antineoplásicos / Técnicas de Cocultura / Células Jurkat / Interferência de RNA / Quimiocina CXCL12 Tipo de estudo: Estudo diagnóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Células da Medula Óssea / Expressão Gênica / Adesão Celular / Células Cultivadas / Células Estromais / Resistencia a Medicamentos Antineoplásicos / Técnicas de Cocultura / Células Jurkat / Interferência de RNA / Quimiocina CXCL12 Tipo de estudo: Estudo diagnóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2005 Tipo de documento: Artigo