Specific targeting cytotoxicity to resistant leukemia cells mediated by anti-Pgp/anti-CD3 diabody / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 342-344, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-255880
ABSTRACT
<p><b>OBJECTIVE</b>To study the specific targeting cytotoxicity to drug-resistant leukemia cells mediated by anti-Pgp/anti-CD3 diabody.</p><p><b>METHODS</b>The diabody was purified by affinity chromatography and identified by SDS-PAGE and FACS. The effect of the anti-Pgp/anti-CD3 diabody mediated lysis of Pgp-expressing tumor cells was assayed by human leukemia nude mice xenograft model in vivo.</p><p><b>RESULTS</b>The diabody was produced in E.coli in a soluble functional form and could bind both Jurkat cells (CD3(+)) and K562/A02 cells (Pgp(+)). The binding rates were 86.25% and 86.26%, respectively. It could inhibit tumor growth by 98.57% and prolonged the survival of mice bearing xenografted K562/A02 cells.</p><p><b>CONCLUSION</b>The diabody was proved to be a potent agent for mediating T lymphocyte cytotoxicity to lyse Pgp expressing tumor cells in vitro and in vivo.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Linfócitos T
/
Complexo CD3
/
Anticorpos Biespecíficos
/
Membro 1 da Subfamília B de Cassetes de Ligação de ATP
/
Resistencia a Medicamentos Antineoplásicos
/
Células Jurkat
/
Ensaios Antitumorais Modelo de Xenoenxerto
/
Citotoxicidade Imunológica
/
Alergia e Imunologia
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo
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