AcMNPV-mediated expression of BmK IT promotes the apoptosis of Sf9 cells and replication of AcMNPV / 生理学报
Acta Physiologica Sinica
; (6): 305-311, 2015.
Article
em En
| WPRIM
| ID: wpr-255945
Biblioteca responsável:
WPRO
ABSTRACT
Chinese scorpion Buthus martensii Karsch (BmK) venom is a rich source of neurotoxins which bind to various ion channels with high affinity and specificity and thus widely used as compounds to modulate channel gating or channel currents. To promote the insecticidal effects of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV), the gene encoding an excitatory insect toxin, BmK IT, was inserted into the genome of AcMNPV to construct a recombinant baculovirus, AcMNPV-BmK IT. Spodopter frugiperda 9 (Sf9) cells were infected with AcMNPV and AcMNPV-BmK IT respectively for 24 h. Results from the MTT assay, TUNEL assay, analysis of the expression level of apoptosis-related proteins (c-Myc, cleaved-Caspase3, Bcl-2 and Bax) of Sf9 cells, the transcription level of key genes (38K, C42, P78, F) of AcMNPV, and viral propagation assay demonstrated that AcMNPV-mediated expression of BmK IT promoted the apoptosis of Sf9 cells and replication of AcMNPV. The results laid a foundation for further structural and functional analysis of BmK IT.
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Assunto principal:
Fisiologia
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Venenos de Escorpião
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Replicação Viral
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Linhagem Celular
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Apoptose
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Nucleopoliedrovírus
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Células Sf9
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Metabolismo
Limite:
Animals
Idioma:
En
Revista:
Acta Physiologica Sinica
Ano de publicação:
2015
Tipo de documento:
Article