Role and mechanism of all-trans retinoic acid in up-regulating apelin expression in vascular smooth muscle cells / 生理学报
Acta Physiologica Sinica
;
(6): 186-192, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-255958
ABSTRACT
This study was aimed to investigate the mechanism of all-trans retinoic acid (ATRA) up-regulating apelin expression in vascular smooth muscle cells (VSMCs). The effect of ATRA on apelin expression in the VSMCs was investigated by RT-PCR, real-time PCR and Western blot analysis. To further define whether retinoic acid receptor α (RARα) mediated the induction of apelin by ATRA, endogenous RARα was down regulated by transfection of siRNA against RARα (si-RARα) or RARα was over-expressed by infection of the adenovirus vector pAd-GFP-RARα in the VSMCs. The results showed that ATRA significantly induced apelin expression in a time- and dose-dependent manner in the VSMCs. Although RARα expression was increased in a time-dependent manner, the expressions of RARβ and RARγ were little changed by the ATRA treatment. When VSMCs were treated with a RARα antagonist Ro 41-5253 prior to the addition of ATRA, or si-RARα was used to down regulate endogenous RARα expression, the blockade of RARα signaling partially reduced the response of apelin to ATRA. Moreover, RARα over-expression, induced by infection of pAd-GFP-RARα, further increased the induction of apelin by ATRA. In conclusion, ATRA may up-regulate apelin expression in VSMCs, and the mechanism may be RARα dependent.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tretinoína
/
Benzoatos
/
Transfecção
/
Transdução de Sinais
/
Regulação para Cima
/
Regulação da Expressão Gênica
/
Cromanos
/
Receptores do Ácido Retinoico
/
Biologia Celular
/
Miócitos de Músculo Liso
Idioma:
Chinês
Revista:
Acta Physiologica Sinica
Ano de publicação:
2015
Tipo de documento:
Artigo
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